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(CHEM 483L) Biochemistry Methods Lab – Proteins

Laboratory methods used in protein biochemistry – protein expression, extraction, purification, separation and analysis. The lab provides hands-on experience with bacterial transformation and culture, protein extraction and chromatography, centrifugation, spectrophotometry, SDS and Native gel electrophoresis and analysis.

Credit Hours 1.0 Lab
Prerequisite CHEM 351L
Co- or Prerequisite CHEM 481
Offered Fall, Spring
Programs -

Course Learning Outcomes

The objectives of this biochemistry laboratory course(aka CLOs) are to help students learn and apply a variety of fundamental and essential protein-based laboratory techniques. To do so, the students will be required to express, purify, quantify, and identify the recombinant green fluorescent protein (GFP).