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(BIOL 441L) Molecular Biology Laboratory

This course provides hands-on training in laboratory techniques widely used in molecular biology: DNA electrophoresis, restriction digestion and ligation, bacterial transformation, DNA extraction and purification, PCR, DNA recombination, expression, sequencing and others.

Credit Hours 1.0 Lab
Pre- or corequisites BIOL 441
Offered Fall
Programs Biology (BS)

Course Learning Outcomes

  1. Demonstrate proficiency in fundamental nucleic acid laboratory techniques by performing DNA design, amplification, digestion, ligation, transformation, purification, and sequencing using established biomolecular methods.
  2. Apply appropriate laboratory instrumentation and safety practices to study and manipulate nucleic acids, including the use of autoclaves, thermocyclers, electrophoresis systems, incubators, imaging systems, and DNA sequencing and analysis software.
  3. Analyze experimental data derived from nucleic acid techniques, including gel electrophoresis, clone screening, and DNA sequencing, to evaluate experimental success and data quality.
  4. Communicate biomolecular experimental results clearly and professionally through accurate laboratory notebook records and formal written lab reports that present data, figures, and conclusions according to scientific conventions.